Affiliated with Université Laval & CERVO Research Centre

Resolution enhancement in confocal microscopy using Bessel-Gauss beams.

TitleResolution enhancement in confocal microscopy using Bessel-Gauss beams.
Publication TypeJournal Article
Year of Publication2017
AuthorsThibon L, Lorenzo LE, Piché M, De Koninck Y
JournalOpt Express
Volume25
Issue3
Pagination2162-2177
Date Published2017 Feb 06
ISSN1094-4087
Abstract

Laser scanning microscopy is limited in lateral resolution by the diffraction of light. We show that we can obtain twenty percent improvement in the resolution of confocal microscopy using Bessel-Gauss beams with the right pinhole size compared to conventional Gaussian beam based confocal microscopy. Advantages of this strategy include simplicity of installation and use, linear polarization compatibility, possibility to combine it with other resolution enhancement and superresolution strategies. We demonstrate the resolution enhancement capabilities of Bessel-Gauss beams both theoretically and experimentally on nano-spheres and biological tissue samples without any residual artifacts coming from the Bessel-Gauss beam side lobes with a resolution of 0.39λ. We also show that the resolution enhancement of Bessel-Gauss beams yields a better statistical colocalization analysis with fewer false positive results than when using Gaussian beams. We have also used Bessel-Gauss beams of different orders to further improve the resolution by combining them in SLAM microscopy (Switching LAser Modes : Dehez, Optics Express, 2013) achieving a resolution of 0.2λ.

DOI10.1364/OE.25.002162
Alternate JournalOpt Express
PubMed ID29519064