Affiliated with Université Laval & CERVO Research Centre

A multimodal micro-optrode combining field and single unit recording, multispectral detection and photolabeling capabilities.

TitleA multimodal micro-optrode combining field and single unit recording, multispectral detection and photolabeling capabilities.
Publication TypeJournal Article
Year of Publication2013
AuthorsDufour S, Lavertu G, Dufour-Beauséjour S, Juneau-Fecteau A, Calakos N, Deschênes M, Vallée R, De Koninck Y
JournalPLoS One
Volume8
Issue2
Paginatione57703
Date Published2013
ISSN1932-6203
KeywordsAluminum, Animals, Glass, Light, Luminescent Proteins, Male, Mice, Microelectrodes, Molecular Probes, Neurons, Optical Fibers, Rats, Single-Cell Analysis, Spectrometry, Fluorescence, Staining and Labeling
Abstract

<p>Microelectrodes have been very instrumental and minimally invasive for in vivo functional studies from deep brain structures. However they are limited in the amount of information they provide. Here, we describe a, aluminum-coated, fibre optic-based glass microprobe with multiple electrical and optical detection capabilities while retaining tip dimensions that enable single cell measurements (diameter ≤10 µm). The probe enables optical separation from individual cells in transgenic mice expressing multiple fluorescent proteins in distinct populations of neurons within the same deep brain nucleus. It also enables color conversion of photoswitchable fluorescent proteins, which can be used for post-hoc identification of the recorded cells. While metal coating did not significantly improve the optical separation capabilities of the microprobe, the combination of metal on the outside of the probe and of a hollow core within the fiber yields a microelectrode enabling simultaneous single unit and population field potential recordings. The extended range of functionalities provided by the same microprobe thus opens several avenues for multidimensional structural and functional interrogation of single cells and their surrounding deep within the intact nervous system.</p>

DOI10.1371/journal.pone.0057703
Alternate JournalPLoS ONE
PubMed ID23469053
PubMed Central IDPMC3585187
Grant ListMOP 12942 / / Canadian Institutes of Health Research / Canada
NS054840 / NS / NINDS NIH HHS / United States
NS064577 / NS / NINDS NIH HHS / United States